The effective dose of phytoestrogen extracted from bean plant on osteoblast | ||
| journal of kerbala university | ||
| Article 1, Volume 10, Issue 2, July 2014, Pages 135-142 PDF (0 K) | ||
| Abstract | ||
| This study was conducted to determine the impact of effective dose ED50 estrogenic plant (phytoestrogen)) extracted from the beans, white beans dry on the effectiveness of osteoblasts Osteoblast cells (in vitro). Through study some parameters genetic and some standards Plant estrogens extracted from white kidney beans, and then extracted effective dose ED50-estrogenic plant, designed the experiment and that Ba for the distribution of random for 48 of the female rabbits into six groups (8 / group) were injected into the first group with saline and used a control (G1) with injected intramuscularly second group 0.07 mg / kg of plant estrogens (G2) and the third 0.1 mg / kg (G3) and fourth 0.2 mg / kg(G4 and the fifth 0.5 mg / kg (G5) and sixth 0.7 mg / kg (G6) daily for four weeks then was measured some occupational standards (such as total cholesterol Total Cholesterol (TC) , high-density lipid protein High lipoprotein density (HDL) and identified the effective dose was 0.2 mg/ kg .Been studying the biological activity of bone cells after estrogen treatment plant at the cells osteoblasts, has been influential estimate the concentration of estrogen on bone and vital cell concentrations using upward (400,200,100,50,25,12.5, micrograms / ml) for 24, 48 and 72 hours. found a concentration of 50 micrograms is the most effective and less toxic. But the study showed that the effect of dose of 25 micrograms was the most effective in activating genes that produce collagen. As for the study of the effect of plant estrogens on the effectiveness of genes collagen and fibronectin observed increase of the activity of bone cells, a collagen, a gene responsible for a building material intra-tissue bone, as well as some of the genes responsible for a building cell membrane proteins, which works on the adhesion of bone cells to Article interfaces Fibronectin and that these genes reduces the process of programmed cell death . | ||
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