SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT | ||
| Basrah Journal of Veterinary Research | ||
| Article 1, Volume 13, Issue 1, June 2014, Pages 50-59 PDF (0 K) | ||
| DOI: 10.33762/bvetr.2014.88125 | ||
| Authors | ||
| ; Tahir A. Fahid; Talib Ahmed Jaayid | ||
| Abstract | ||
| The present study included determinationof fetal sex prenatal by analyzing free fetal DNA cells in maternal plasma by using dupplex PCR . At the first amplifying the DNA which extracted from male and non-pregnant female goat plasma which used as control to confirm the accuracy of this technique. After thatblood were collected from 5 pregnant females goat prepared the plasma and extract the DNA by using heat-based direct method for plasma and then investigate the sequences GAPDH and DYS14 through the use of dupplex polymerase chain reaction technique and the results were 2 (40%) males show a double bands one at 97 bp representing GAPDH and the second at 198 bp representing DYS14 and 3 (60%) females show a single band at 97 bp representing GAPDH. This technique of sexing is applied for the first time on animals especially in Iraq. | ||
| Keywords | ||
| Fetal sex; goat; Plasma; Duplex PCR | ||
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