Extraction, purification and characterization of lipoxygenase from Pleurotus ostreatus. | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 55, Issue 1, March 2014, Pages 61-69 | ||
| Authors | ||
| Qabass L. Abdullah; Mona H. AL-Jibori; Sanad B. AL-Arrji | ||
| Abstract | ||
| Lipoxygenase was extracted from the cup of Pleurotus ostreatus ( Jaq : Fr ) oyster mushroom for the first time in Iraq, and purified homogeneously through precipitation with 40% saturation of (NH4)2SO4 as a partial purification then loaded on DEAE-Cellulose (Diethyl amino ethyl Cellulose) ion-exchange chromatography column and then the highly active elution parts have been passed through gel filtration column with Sephacryl S-300 as a final purification with 804 (U/mg protein) specific activity, 11.32 fold of purification and 36.54% yield . The molecular weight of the enzyme was estimated to 74 KDa by gel filtration Sephacryl S-300 column and the isoelectric point for enzyme was 5.3. The optimal pH for lipoxygenase activity and stability were 8 and 6-8.5 respectively, and the optimal temperature for the activity and stability of the enzyme were 30 and 10-45 respectively. Also the activation energy necessary for Lipoxygenase to convert lionleic acid to product and for enzyme denaturalization were calculated to 9.674 and 28.087 Kilo calorie/mole respectively. | ||
| Keywords | ||
| Pleurotus ostreatus; Oyster Mushroom; Purification; Lipoxygenase | ||
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