Alpha - amylase immobilize which produced from malt of local barley on calcium alginate | ||
| Basrah Journal of Agricultural Sciences | ||
| Article 18, Volume 25, Issue 1, June 2012, Pages 149-160 PDF (0 K) | ||
| DOI: 10.33762/bagrs.2012.56194 | ||
| Author | ||
| Dhia F. Alfekaiki | ||
| Abstract | ||
| The study included, Alpha - amylase immobilized which was produced from malt of local barley by four different binding materials. The best one was calcium alginate, which gave highest binding for protein (9.25 mg /gm) and highest efficiency of enzyme immobilization 68%. of Calcium alginate . Best concentration of Calcium alginate to bind enzyme was 4% which gave an enzyme activity of 160 unit/ g and good hardens Volume of 2ml was chosen as the best volume of calcium alginate to bind alpha – amylase. Increasing of binding enzyme from 0.5 -3 g was found to result in an showed a gradually increase in enzyme activity at a gradually and gave highest activity at a quantity of 3 g of enzyme binding calcium alginate. Enzyme –binding calcium alginate was found to retain 98.5% of alpha – amylase activity when using it 6 folds. Effect of amount of enzyme – binding calcium alginate on starch solution viscosity was found to decrease gradually after 5 minutes from the binding of the reaction, the relative viscosity reached 70% then 60 min from the start of the reaction. | ||
| Keywords | ||
| Immobilization of enzymes; Alpha amylase | ||
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