IN VITRO MICROPROPAGATION OF POINSETTIA (EUPHORBIA PULCHERRIMA WILLD.) | ||
| Kirkuk University Journal For Agricultural Sciences | ||
| Article 1, Volume 3, Issue 1, June 2012, Pages 1-9 PDF (753.29 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.58928/ku12.03113 | ||
| Authors | ||
| Layla S. M. Al-Mizory; Rafail S. Toma | ||
| Abstract | ||
| A rapid, efficient and reproducible propagation protocol was developed for initiation, multiplication and root formation of poinsettia (Euphorbia pulcherrima Willd.) through the culture of single nodal segments on basal MS medium. The results showed that the best cultures were established on solid MS medium supplemented with (5.0+0.1) from both BA and NAA respectively after being surface disinfested in %07 ethyl alcohol for 00 seconds, followed by immersion in mercuric chloride (0.57) for five minutes. The best shoots multiplication was achieved with the combination treatment 5.0+5.0 mg/l of BA and NAA (6.16 shoots/ explant) as compared with the use of BA or Kinetin separately. The rooting percent (5007) was recorded by adding 0.0 and 0.0 mg/l IBA. IBA performance was better than IAA in producing higher number of roots by giving 6.60 roots/ explant on shoots cultured on solid MS medium supplemented with 0.0 mg/l IBA. A high survival rate (%07) was found with poinsettia plantlets gradually acclimatized into the out-air conditions. | ||
| Keywords | ||
| Poinsettia; Euphorbia pulcherrima Willd; micropropagation; In Vitro | ||
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