Estimation of Gene Expression by Differential Display Technique of Potato Cultivars (Solanum Tuberosum L.) Grown in vitro Under Drought Stress | ||
| IRAQI JOURNAL OF DESERT STUDIES | ||
| Article 1, Volume 13, Issue 2, December 2023, Pages 1-9 PDF (716.35 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.36531/ijds.2023.141560.1041 | ||
| Authors | ||
| Tuqa R. Noor1; Radhi Dheyab ِِAbed* 2; Shatha A. Yousif3 | ||
| 1Seed Inspection and Certification Directorate, Ministry of Agriculture, Baghdad, Iraq | ||
| 2University of Fallujah, College of Applied Sciences | ||
| 3Agricultural Research Directorate, Science and Technology, Baghdad, Iraq | ||
| Abstract | ||
| A laboratory experiment was carried out at Genetic Engineering Department- Agricultural Research Directorate/ Ministry of Science and Technology for the period between March 2019 and May 2020. The aim was to characterize the gene expression at different rates of drought stress. Stem cuttings were cultured in MS medium which contained three concentrations of Poly Ethylene Glycol (PEG) as a stress factor (0, 0.5 and 2.5 %). Differential Display Reverse Trancrptase- DDRT was applied to study the genetic markers related with drought tolerance by using 6 randomized primers (B5, D2, E12, F12, Q16 and H9). The experiment included seven genotypes of potatoes, four of which were certificated in Iraq, namely Arnova, Burren, Provento and Riviera and the rest were brought from the International Potato Institute in Peru which are 58, 60 and 67. All primers (6 primers) gave a polymorphism, with a total of 101 bands. Maybe this indicate the existence of gene expression in plants that subjected to drought stress. The primers varied in number of bands, the lowest number of bands were in D2 which gave 15 bands while the highest number of bands (18 bands) were in B5, F12 and H9. A number of bands of all primers studied were observed in plants under control and drought stresses, indicating the possibility of these DNA fragments involved in drought tolerance. | ||
| Keywords | ||
| polymorphism; differential display; potato; gene expression; bands | ||
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