Morphological and Molecular Identification of Adoretus hirsutus (Ohaus, 1914) (Coleoptera:Scarabaeidae:Rutelinae) from Erbil Governorate Kurdistan Region- Iraq | ||
| Kirkuk University Journal For Agricultural Sciences | ||
| Volume 14, Issue 3, September 2023, Pages 296-305 PDF (1.18 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.58928/ku23.14331 | ||
| Authors | ||
| Gona S. Sharif* 1; Abbas M. Farage2; nabeel abdulakadir mawlood3 | ||
| 1Plant Protection Department , College of Agriculture, University of Salahaddin-Erbil, Iraq | ||
| 2College of Pharmacy Erbil, Hawler Medical university Erbil, Iraq | ||
| 3Plant Protection Department, College of Agricultural Engineering Science, Salahaddin University– Erbil, Iraq | ||
| Abstract | ||
| This study provides a detailed description of Adoretus hirsutus (Ohaus, 1914) (Coleoptera:Scarabaeidae: Rutelinae), as a first record in Iraq. Between March and July 2022, we collected specimens from various weed flowers in different locations within the Erbil Governorate, Kurdistan Region, Iraq. According to molecular analysis, Adoretus hirsutus was used as a source of samples for PCR amplification of the fragments (710 bp) of the mtCOI gene for phylogenetic analysis. To compare the nucleotide sequence with those of other insect species, a section of the mtCOI gene from the collected insect was aligned with the NCBI GenBank database using the BLAST tool. The BLAST results showed that the second record in the NCBI GenBank identity of insects. The COI sequence of Adoretus hirsutus was submitted to GenBank with accession numbers OQ4288117, OQ428818, and OQ428819. The morphological diagnostic characteristics of the species are; Labrum nearly cup shaped, lateral margins moderately concave with a row of small spines, median apical projection overhanging the mentum. Mandibles irregular shaped, apically with single oval shaped tooth. Terminal maxillary palpomere 1.5 times as long as the 2nd. Antenna brown, consisting of 10 antennomeres ending in a unilateral three lamellated club, equal in length. Outer margin of fore tibia with three acute teeth. Aedagaeus moderately curved, parameres elongated oval, apical part is very acute. Some important parts such as labrum, mandible, antenna, fore legs, elytra, pygidium and male genitalia have been photographed. | ||
| Keywords | ||
| Molecular identification; morphological study; mt COI gene; Adoretus hirsutus; sequencing | ||
| References | ||
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