Production of synthetic seeds in almond plan Prunus amygdalus (local type) In Vitro | ||
| Kirkuk University Journal For Agricultural Sciences | ||
| Article 12, Volume 9, Issue 4, December 2018, Pages 88-101 PDF (1.36 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.58928/ku18.09412 | ||
| Authors | ||
| Wisam Khazaal Khalid; Ammar Zeki Ameen Kassab | ||
| University of Mosul - College of Agriculture | ||
| Abstract | ||
| study was conducted to investigate the possibility of producing synthetic seeds of almond Prunus amygdalus L, plant (local type) by encapsulated shoot tips and single node (2-4 mm) as explants with different concentrations (1, 2, 3, 4 and 5%) of sodium alginate (SA) and then evaluate its ability to conversion to plantlets after stored in the dark at 4 ° or 25 ° C for 1, 2 and 3 months, The results showed after four weeks of planting stored seeds on MS solid medium free of growth regulators, seeds coated with concentration of 4% sodium alginate was moral superiority in the percentage of seeds conversion into plantlets comparing with other coated seeds and other different concentrations studied which gave conversion ratios reached to 46,67% and 36,67% for each of the shoot tips and single node respectively, On the other hand outperformed seeds stored at 4 ° C to those stored at room temperature (25 m °) which gave conversion ratios reached to 35% and 22,5% of the seeds produced by the shoot tips and to 27,5% and 19,17% of the seeds produced from the single node، The study also showed decrease of the conversion percentage of seeds with the length of the storage period، reaching the highest 36,25% and 32,5% for each of the seeds produced from the shoot tips and single node which stored for a month and the lowest 21,25 to the seeds produced from the shoot tips which stored for two months، and 17,5 for the seeds which produced from single node stored for a period of 3 months With regard to the interaction treatments between the studied factors, the study showed that the seeds which produced by encapsulated shoot tips with 4% sodium alginate gave the highest percentage of 80% and conversion when stored at 4 ° for 3 months، | ||
| Keywords | ||
| Almond; Synthetic seeds; In Vitro | ||
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