Molecular and Serological Detection of Toxoplasma gondii in Random Sample of pregnant and aborted women of Nineveh | ||
| College Of Basic Education Research Journal | ||
| Article 78, Volume 17, Issue 2, July 2021, Pages 1848-1864 PDF (1.34 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/berj.2021.168580 | ||
| Authors | ||
| Najah Subhi Nayef Khatab Al-Omer1; Aliaa Hussein Ali Muhsin Al-Marsoomy2 | ||
| 1University of Mosul College of Science Department of Biology | ||
| 2Department of Biology College of Sciences University of Mosul | ||
| Abstract | ||
| The present study is to identify the rate of parasitic disease of toxoplasmosis among among females from 120 pregnant and aborted women attending to the Department of Obstetrics and Gynecology of the AL-Kansaa, AL- Salaam Hospital and 2 private clinics during (September 2019-febrauary2020). Three methods of tests to search for toxoplasmosis latex test, combo IgG and IgM rapid cassette and finally by PCR. Questionnaire is applying by pregnant and aborted patients and according to the information the females was divided to groups involved age, career, gestational period, housing cat or not in living zone ,number of abortion ,blood group and according to residence. The results show big rate of toxoplasmosis by latex test the positive cases 62(62%).the result re-tested by another serological method combo IgG and IgM rapid cassette and give the result 44 (73.33%) and 6 (9.09%) for IgG and IgM respectively. The result was confirmed by third test using molecular test conventional PCR to show the result 32 (51.61%). We used EDTA tube for whole blood for PCR, gel tube for serological testes. Three methods of tests to search for toxoplasmosis latex test, combo IgG and IgM rapid cassette and finally by PCR. | ||
| Keywords | ||
| : Molecular detection; Toxoplasma gondii; pregnant and aborted; serological tests; latex test; Rapid test; IgG and IgM | ||
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