Effect of Irradiation and Cholesterol and Progesterone on Cardiac Glycoside Production From Shoots of Digitalis lanata Plant In Vitro. | ||
Journal of Kerbala for Agricultural Sciences | ||
Article 11, Volume 2, Issue 3, September 2015, Pages 122-135 PDF (772.52 K) | ||
Authors | ||
Sarab AB Al-Mokhtar1; Mohammed SH Hamed2 | ||
1College of AgricultureUniversity of Kerbela | ||
2College of AgricultureUniversity of Baghdad | ||
Abstract | ||
Seeds of foxglove (Digitalis Lanata) were irradiation with (0,30) gray of gamma ray and sown in sold MS media ,after one month shoot tips 2 cm were taken and cultured on MS media provide with 2 mg/l BA and either cholesterol or progesterone at 0,1,2,3mg/l of each, progesterone at 2mg/l gave the highest values of Lanatoside, Digoxin, Digitoxin and Gitoxin were(54.89, 67.62, 106.66 and 88.14) µg/g of dry weight respectively and highest content of chlorophyll and carbohydrates were 1.92 and 2.67mg/g respectively. Irradiation with 30 gray improved the characters test, it gave(66.15, 72.87, 128.67, 94.31) µg/g of the cardiac glycosides respectively | ||
Keywords | ||
Digitalis; In Vitro; Gamma Ray; Cholesterol; Progesterone; cardiac glycoside; dry weight | ||
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