Mohammed Jihad, R. (2012). Simultaneous determination of tryptophan and tyrosine in binary mixture by zero-crossing second derivative spectrophotometry.. Alustath, 4(1), 35-39. doi: 10.37652/juaps.2010.15419
Riyadh Mohammed Jihad. "Simultaneous determination of tryptophan and tyrosine in binary mixture by zero-crossing second derivative spectrophotometry.". Alustath, 4, 1, 2012, 35-39. doi: 10.37652/juaps.2010.15419
Mohammed Jihad, R. (2012). 'Simultaneous determination of tryptophan and tyrosine in binary mixture by zero-crossing second derivative spectrophotometry.', Alustath, 4(1), pp. 35-39. doi: 10.37652/juaps.2010.15419
Mohammed Jihad, R. Simultaneous determination of tryptophan and tyrosine in binary mixture by zero-crossing second derivative spectrophotometry.. Alustath, 2012; 4(1): 35-39. doi: 10.37652/juaps.2010.15419

Simultaneous determination of tryptophan and tyrosine in binary mixture by zero-crossing second derivative spectrophotometry.

Journal of University of Anbar for Pure Science
Article 12, Volume 4, Issue 1, April 2010, Pages 35-39    PDF (336.13 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2010.15419
Author
Riyadh Mohammed Jihad*
Women Education College, Al-Anbar University
Abstract
Rapid and accurate binary mixture resolution of tryptophan and tyrosine was performed. Differential-derivative spectrophotometry with a zero-crossing measurement technique was used for the quantitative determination of tryptophan and tyrosine in laboratory-prepared mixtures. Neither sample pretreatment nor separation were required. Linear calibration graphs of differential second derivative values were (at 222.4 and 217.9 nm for tryptophan and tyrosine , respectively) versus concentration in the ranges 0.1–20.0 and 1.0– 50.0 µg ml_1, and the linearity was satisfactory (r = 0.9987 and r = 0.9997), for tryptophan and tyrosine , respectively) were obtained .The relative standard deviations were found to be less than 1.03%, indicating reasonable repeatibility of method.The results obtained by the proposed method has been statistically compared by means of Student t-test and by the variance ratio F-test where shows a good agreement .
Keywords
tryptophan; tyrosine; spectrophotometry; Simultaneous determination; zero; Crossing
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