Identify some of the Genetic Markers and gene Ttaavatha CAPN1 and its relationship with some of the qualitative Characteristics before and after slaughter of Broiler Chickens Rose 308 | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 9, Issue 1, June 2016, Pages 109-122 | ||
| Authors | ||
| O. M. Abdullah; A. A. Abbas; R. S. Ramadhan | ||
| Abstract | ||
| A total of one hundred birds of commercial broiler (Ros 308) were selected randomly from a private flock at the age of 42 days. Samples of blood were collected from each bird individually then birds were slaughtered. The genetic analyses was performed in the laboratories of College of Science, University of Al-Nahrain, The aim of extract DNA to identify genotypes (Genotype) gene CAPN1. The study aimed to find the relationship of these genes (genetic markers) some important qualities of sacrifice (slaughter weight, the weight of the main Alqtaaat, edible viscera weight, dressing percentage, abdominal fat weight) in broilers under study Knowing repeating and repeat genotypes and gene sequences to gene CAPN1 To improve the qualities the most important economic to broilers Meat. The results could be summarized as following: gene were determined in the broilers of the current study depending on the bands that got it from the electrophoresis of the individuals DNA using RFLP technique and restricted enzyme with (Bam H1) and the genotypes were determined according to the size of the parts of the gene for gene CAPN1. Three types of genotypes for the CAPN1 gene were determined according to the flock size, where AA, AB and BB. The genotype frequency of the mentioned genotypes were 0.25, 0.55 and 0.20 respectively and the gene frequency was 52.5% A and 47.5% B. The genotype BB of the CAPN1 gene was superior significantly (P<0.01) as compared with others genotypes concerning live body weight, carcass weight, thigh weight, gizzard, and the ratio of upper thigh to the breast width. The correlation coefficients between CAPN1 gene and each of the live body weight, carcass weight and breast weight were significant and positive. The genotypes BB for the CAPN1 are the distinct as they superior significantly (P<0.05) compared with others genotypes due to the polymorphism of these gene, hence, we recommend the using as genetic markers in the selection programs. The mutations were determined in CAPN1 gene as a result of studying the sequences that indicate the occurrence of mutations for the Substitution and deletion types (37.5%) while 25% did not show any change in the broilers samples | ||
| Keywords | ||
| CAPN; PCR; RFLP; Sequencing; Broiler Chickens | ||
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