Production and characterization of Exopolysaccharid(EPS) from local isolate Lactobacillus Plantarum and test the biological Activities | ||
| journal of kerbala university | ||
| Article 1, Volume 12, Issue 0, January 2016, Pages 104-115 PDF (0 K) | ||
| Abstract | ||
| Exopolysaccharid (EPS) Produced by local isolate Lactobacillus spp. which selected among 45 bacterial isolates producing this polymer represented by Lactobacillus spp. , Bacillus spp. and Psedomonas spp. the isolate has been identified by biochemical test and the ability fermented sugars as lactobacillus plantarum . After production two steps were used for partial purification of the polymer as follows, the first step by using Trichloroacitic acid (TCA) solvent and the second step by dialysis the yields were(77.4 and 63.8)% for the two purification steps respectively.The polymer was characterization by using Fourier Transform Infrared Spectroscopy (F.T.I.R) with molecular weight 2.3 * 105 Dalton by using viscometer and showed two spots when separated by Thin Layer Chromatography (TLC) which is identifide as a glucose and galactose. The polymem showed antibacterial activity against some types of pathologenic bacteria Proteus spp.,Bacillus cererus and Stenotrophomonas spp.with an inhibition zone diameter of ( 23,21 and 19 ) mm respectively.While, there was no inhibitory effect against fungi. Antioxidant activity has also been tested,where IC50 value against ABTS free radical was at 100 Mg / ml. | ||
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