The E ffect of Aqueous Albizia Flowers Extract Albizia lebbeck on Production of Certain Enzymes from Salmonella typhi | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 10, Issue 1, June 2016, Pages 25-30 | ||
| Authors | ||
| Ali J. R. AL-Sa; ; Lamees M.R. Abbas | ||
| Abstract | ||
| Salmonella typhi is Gram-negative and pathogenic bacteria, its produces variety of enzymes for growth. The present study aimed to study the effect of aqueous albizia flower extract 10 % on production of three enzymes from Salmonella typhi, and the effect of this extract on their activity. The production of amylase and protease enzymes from Salmonella typhi, were increased after addition of crude plant extract 10% into media with specific activity 141.3 and 72.6 U/mg respectively, while production of cellulase was decreased after addition, with specific activity 31 U/mg, compare with specific activity before addition of crude plant extract. Aqueous Albizia flowers extract 10% increases specific activity of amylase and protease, where they gave 16 and73.8 U/mg respectively after incubation with plant extract for 30 minutes, while specific activity 6.2 U/mg for cellulase was decreased after this incubation, compare with specific activity before incubation with plant extract. The phytochemicals screening of aqueous Albizia flower extract were positive for alkaloids, steroids, glycosides, flavonoids, tannins, phenols, and saponins, while were negative for terpens, resins, coumarines and anthraquinones. | ||
| Keywords | ||
| Salmonella typhi; amylase; protease; cellulase and Albizia lebbeck flowers | ||
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