Detection of Carbonic Anhydrase Enzyme in Sulcus Median in Hind Brain (Fourth Ventricle) in the Hind Brain of the Adult White Rat (Ruttsrutts) | ||
Ibn Al-Haitham Journal For Pure And Applied Science | ||
Article 1, Volume 27, Issue 3, December 2014, Pages 1-7 | ||
Authors | ||
Thekra A. Ibrahim; Anam R. Al-Salihi; Nahla A. Al-Bakri | ||
Abstract | ||
The method of heavy metals deposition which is based on cobalt in detection of Carbonic anhydrase enzyme in the Sulcus median in the hid brain (fourth ventricle) in the adult white rat (Ruttsrutts). An essential amended in the method has been done by using cobalt chloride (CoCl2) instead of cobalt phosphate (Co3(PO4)2) in the reaction medium. Any efficacy of enzymichistochemical for carbonic anhydrase enzyme did not show in histological sections. The floor of the fourth ventricle of the brain is specific, clearly any histochemical reaction sediments have not been found in sulcus median of the floor of the fourth ventricle. The corresponding stain to green methyl which was observed clearly in sulcus median region. The ventral surface of area postrema composes of differentiated columnar cells without existence for any efficacy for carbonic anhydrase enzyme in it. Area postrema did not show any effective in both outerion region where appears as one mid line component or in back region where as continuing couple with central canal lining appears. | ||
Keywords | ||
area postrema; carbonic anhydrase enzyme; sulcus median | ||
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