STUDYING THE FREQUENCY OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS THROUGH THE MOLECULAR DETECTION OF MECA | ||
| IRAQI JOURNAL OF MEDICAL SCIENCES | ||
| Article 1, Volume 12, Issue 3, December 2014, Pages 289-294 | ||
| Authors | ||
| Rafeef Y. Rasheed; Ahmed S. Abdulamir; Amir H. Raziq | ||
| Abstract | ||
| Background:Methicillin-resistant Staphylococcus aureus is a major cause of serious nosocomial infections and it is very important to have a reliable test to detect these bacteria. mecA encodes the penicillin binding protein 2a, which is associated with staphylococcal methicillin resistance. Objective:The study was to determine the frequency of methicillin-resistant Staphylococcus aureus in different specimens from Iraqi patients and to genetically characterize and type the samples of methicillin- resistant Staphylococcus aureus through the detection of mec A gene. Methods:Sixty clinical isolates of Staphylococcus aureus were submitted to DNA extraction. Genomic DNA was submitted to conventional polymerase chain reaction assays, employing MR1-MR2 primers (primer set). The results were compared to the cefoxitin disks agar diffusion method. Results :Fifty seven of the sixty isolates showed positive results for mecA amplification while three isolates (5%) showed negative results for mecA gene. Conclusion:Good correlation between the mecA gene detection by PCR and the cefoxitin disk diffusion methods was obtained. Key words:mecA gene, Staphylococcus aureus, methicillin resistance Staphylococcus aureus | ||
| Keywords | ||
| mecA gene; Staphylococcus aureus; methicillin resistance Staphylococcus aureus | ||
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