DETERMINATION OF INHIBITION ACTIVITY TO CURCUMA LONGA L. RHIZOME EXTRACTS AND | ||
| IRAQ JOURNAL OF AGRICULTURE | ||
| Article 1, Volume 19, Issue 7, December 2014, Pages 0-0 | ||
| Authors | ||
| L. A. K. Alzubaidi; E. A. Khalaf; M. A. Abdulrazaq; A. S. Selman | ||
| Abstract | ||
| The qualitative chemical test of the active groups in the in the curcumin rhizomes (Curcuma longa L.) that extracted by Hot watery, ultrasonic and ethanolic alcohol. The results showed that the curcumin extracts contain the major active groups, while the extracts were varied with quantitatively and qualitatively in their active groups, and Tannic acid was further analyzed to determine their total phenolic content by using the Folin- Ciocalteau method, the ethanolic extract was exerted highest percent of total phenolic content, and the inhibitor activity of the curcumin plant extracts in some fungi tested strains, which includes Aspergillus niger, Aspergillus Flavus and Fussarium spp. using the agar diffusion method. It was noticed that the inhibitory activity was varied according to the extraction method and solvent employed methods and and the microorganism. The ethanolic extract of curcumin plant at 2.5% and 5% showed significant effect (P ≤0.05) in accordance to the two other extracts in the inhibition of the fungi tested isolates as the diameter of the growth areas of tested fungi isolates was 0, 0.66 and 0 mm, and 1.6, 2.83, 1.86 mm respectively and the Asp. flavus showed higher resistance in comparison to the other tested strains, and the ethanolic extract activity in concentration 1, 0.5 and 0.25% at alone in fungi tested isolates to select the optimums concentration to the activity and appeared with 1% concentration, and when treated with the grains samples that includes yellow maize, rice and pun nut that’s treatment with fungi cells in number 1x106cell/ mm2 for incubation 28 and 36 days at alone at 28 ±1 C° comparison with positive control samples, and Appeared don’t showed fungal growth that or differences in grain samples texture before incubation period for 28 days. | ||
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