EVALUATION OF THE OPTIMAL CONDITIONS FOR CELLULOSE PRODUCTION FROM Acetobacter xylinum FEA 48 ISOLATED FROM VINEGAR | ||
| IRAQ JOURNAL OF AGRICULTURE | ||
| Article 1, Volume 19, Issue 7, December 2014, Pages 0-0 | ||
| Authors | ||
| E.E. Al-shamary; M.O. Mohy- Aldeen; A.H. Aldahan | ||
| Abstract | ||
| The optimal condition for production of cellulose from local isolate Acetobacter xylinum FEA 48, isolated from date syrup vinegar were studied . The obtained results revealed that 4% fructose and 3% glucose were the best carbon sources, the produced cellulose were 4.75 g/l and 2.15 g/l respectively. It was also found that organic nitrogen was the best for producing cellulose, as yeast extract with 0.8% concentration represented best nitrogen source .The productivity was 6.5 g/l and 9.0 g/l using the aforementioned carbon source respectively. The optimum temperature for cellulose production from the isolate was 30c◦, the optimum initial pH was 6.5 and 7 for both fructose and glucose media respectively. It was also found that using 60 ml of media contained fructose and 80 ml of media contained glucose resulted an increase in produced cellulose that valued 6.95 and 4.98g/l respectively. The effect of aeration on cellulose productivity was studied using shaking incubator at 150 RPM, and caused productivity drop for both fructose and glucose and valued 4.5 and 2.2g/l considering the above mentioned optimum condition. The effect of enrichment of production media with organic compounds that were ethanol, acetic acid, malic acid, succinic acid. It was found that 1.2% ethanol resulted high increase in produced cellulose that valued 12.9 and 10.5g/l for fructose and glucose respectively. It was also found that highest productivity was achieved on the fourth day of incubation in fructose media, the increase valued 36%. The optimum productivity with glucose media achieved at the third day of incubation and valued 38.8%. | ||
|
Statistics Article View: 59 PDF Download: 25 |
||