ISOLATION AND IDENTIFICATION OF PHYTOHORMONE PRODUCING BACTERIA FROM RHIZOSPHERE OF SORGHUM (sorghum bicolor) | ||
| IRAQ JOURNAL OF AGRICULTURE | ||
| Article 1, Volume 19, Issue 5, September 2014, Pages 0-0 | ||
| Authors | ||
| K. A. Mhammad; M. M. Taen; A. M. Jwaad; F. M. Amen; M. Jaafar; S. A. R. Mahmood | ||
| Abstract | ||
| This research was aimed to isolate and identify rhizospheric bacteria capable for producing indole acetic acid and indole butric acid. However, quantification of IAA choose the best producing isolate. Two nitrogen free media and modified minimal medium (MS) with tryptophan were used in bacterial isolation. Different bacterial isolates (non spore forming and spore forming) were screened for IAA production. Qualitative screening of bacteria indicated that only six isolate out of thirty two produced red zone around the bacterial growth after treatment with Salkawski's reagent. The radius of zone reached to 30 mm with KM2 isolate culture, whereas, five non spore forming isolates and two spore forming isolates produced red zone appeared restricted to the growth. Quantitative determination indicated that KM2 produced 15 microgram/ml IAA followed by B2 which produced 11 microgram/ ml. The identification tests indicated that the isolate KM2 is belong to Pseudomonas spp and B2 to Bacillus spp. Analysis by HPLC depending on standard phytohormones showed that indole acetic acid (IAA) and indole butric acid (IBA) were produced by both isolates. | ||
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