EXPRESSION OF P4502E1 phytoremediation GENE IN Arabidopsis thaliana AND Sesbania grandiflora | ||
| IRAQ JOURNAL OF AGRICULTURE | ||
| Article 1, Volume 19, Issue 5, September 2014, Pages 0-0 | ||
| Authors | ||
| R.S. Mohammad; K.M. Ibrahim; N.A. Ali; A. Al-Dawood | ||
| Abstract | ||
| The present study was conducted with the aim of genetic modification of the commonly grown Sesbania grandiflora plant in many regions of the world including Iraq. Phytoremediation is the use of plants for the remediation of contaminated land mainly in bearing plants and pollutants, which means the ability of plants to tolerate or assemble high concentrations of toxic or harmful substances whether they are organic or inorganic in their tissues without affecting their life cycle in order to produce tolerant plants. Two plant species were subjected to genetic modification in this study namely, Sesbania grandiflora and Arabidopsis thaliana. The bacterium victor Agrobacterium tumefacies was used to transform the two plant species with the cytochrome gene P4502E1 which has been isolated from rabbit liver. The presence of the gene was investigated in plants using polymers chain reaction (PCR) after designing primers for this purpose to detect the presence and expression of the transferred genes. Transformation was proved after gene expression of P4502E1. Results showed generation of a new metabolic pathway in the genetically modified plants towards their ability in degrading many environmental pollutants. The current work has built the principles for S. grandiflora A. thaliana genetic modification and propagation in vitro which facilitates the processes of genetic modification in the future for these plants with a new plant genes and apply this technology to other plants. This approach will lead to reduce land reclamation cost much less than traditional methods, in addition to other advantages such as lowering atmospheric temperatures, increasing vegetation and improving the overall environment for aesthetic, health and social considerations. | ||
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