ENHANCEMENT OF SECONDARY METABOLITES IN CULTURED PLANT CELLS OF TOMATO THROUGH STRESS STIMULUS | ||
| IRAQ JOURNAL OF AGRICULTURE | ||
| Article 1, Volume 19, Issue 5, September 2014, Pages 0-0 | ||
| Authors | ||
| F. M. K. Al-Dabbagh; H. I. Mohamed; M. S. Hamad | ||
| Abstract | ||
| An experiment was conducted to study the effect ofsucrose, glucose, polyethylene glycol 6000in the amount of secondary metabolites extracted from the callus induced from cotyledenous leaves for bothtomato hybrids: Shorouk and GS-12. The results showed that the addition of sucrose at concentration of 60 g/l to the MS medium gave the highest value of phyton amounted to 1438.13 and 118.76 μg/gcallus dry weighof hybrids: Shorouk and GS-12, respectively, also it increased production of Lycopene and β-carotene in Shorouk callus reached 849.78 and 203.79 μg/g respectively, while the concentration 90 g/l increased the production of Lycopene and β-Carotene in callus ofGS-12 was 268.63 and 204.68 μg/g,respectively. As for the glucose, 60 g/l has given the higher amount of phyton amounted to 98.88 μg/gcallus dry weigh of Shorouk, while influenced the addition of30 g/l in the production of phyton, which amounted 585.12 μg/g for GS-12 hybrid. Adding of 90 g/l of glucose to the medium was more effect onLycopene productionreached 479.37 and 375.33 μg/gfor Shorouk and GS-12 hybrids, respectively, and the highest amount of β-Carotene reached 302.63 and 271.96 μg/g callus dry weighof both hybrids, respectively. The results indicate that the addition of 60 g/l PEG 6000 was the most influential in increasing the production of phyton, Lycopene and β-carotenein callus dry weigh of Shorouk, it was 35.41, 499.62 and 167.30 μg/g, respectively, and it stimulated the production of β-carotenein callus of GS-12which amounted to 319.24 μg/g, while MS media containing 90 g/l of PEG 6000increased the production quantity of phyton and Lycopenein callus dry weigh of GS-12 which amounted to 65.62 and 491.84 μg/g respectively. | ||
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