Micro propagation of four Potato Cultivars Solanum tuberosum L. in vitro | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 8, Issue 4, December 2014, Pages 19-24 | ||
| Authors | ||
| Zainab Abduljabar Hussain AL-Hussaini; Shatha AyedYousif; Shymaa Abdullateef Musa; Nora Sahib Abd; Tagreed Abduljabar; Hayfa Muhsen | ||
| Abstract | ||
| Vegetative buds excised from tubers for four varieties of potatoes namely: Arnova, Brovento, Rivira and Bureen (after breaking the dormancy phase) which sterilized with 2% Sodium hypochlorite for 10 minutes and cultured in the MS medium supplemented with 0.5 mg/l of IAA or IBA with different concentration of BA, as well as control treatment in the initiation stage, while different concentrations of BA (0.0, 0.5, 1.0, 1.5, 2 mg/l) with 0.5 IAA mg/l in multiplication stage. All cultures incubated at a temperature of 2 ± 25º C and an illumination of 1000 lux for 16 hours/ day. Data of mean number of branches shoot per plant and their length and leaves for two stages were investigated. The results showed that BA + IAA at 0.5 mg/L for each one, gave the highest initiated percentage, shoot per plant ,length, reached 75%, 1.5 shoot / bud, 2.21cm and 3.88, leaf/ bud, respectively. Results of multiplication indicated that there was a significant interaction effect of both cultivars and BA concentration on all characterizes, Based on this interaction, Arnova cultivars at 1.5 mg/L BA showed the highest mean of shoot per plant (4.0) While Rivera cultivars at 0.5 mg/L BA gave highest mean of length, leaves per plant reached 6.45cm,7.75 leaf/plant respectively. | ||
| Keywords | ||
| Potato; imitation and multiplication; IAA; IBA; In Vitro | ||
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