Investigation the role of fetal calf serum on the viability of gonad cells cultured in vitro for fresh water snail bellamya bengalensis | ||
| journal of kerbala university | ||
| Article 1, Volume 10, Issue 2, July 2014, Pages 270-274 PDF (0 K) | ||
| Author | ||
| Ali Abdul-Hussain Ghazzay | ||
| Abstract | ||
| The present study conducted to investigate the role of fetal calf serum (FCS) on the viability in vitro cultured gonads cells of fresh water snail bellamya bengalensis and determine the optimal concentration of (FCS) for gonads cell cultured in vitro. the fresh water snails bellamya bengalensis were collected from Shamyiah river-Dewanyah province, the snail were dissection out to obtain the gonad cells ,which cultured in (M199 medium) medium supplemented with different concentration of fetal calf serum (10%,20%),the gonad cells were cultured in (M199 medium) supplemented with (FCS) revealed higher viability rate compare with basal medium(non-supplemented with FCS) and control ,while the medium supplemented with (20% FCS) had more positive effect on the viability on the viability of gonad cells than the medium supplemented with(10% FCS). FCS had positive effect to maintain the viability of gonad cells for bellamya bengalensis in vitro cultured . | ||
| Keywords | ||
| FCS; medium | ||
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