Thermostability Of Cellulases Produced By The Actinomycete Bacteria Thermomonospora fusca | ||
| Al-Ma'mon College Journal | ||
| Article 1, Volume 0, Issue 23, July 2018, Pages 239-250 | ||
| Author | ||
| Ala Sharif Abbas | ||
| Abstract | ||
| Thermostability of crude extracellular endoglucanase, exoglucanase and β- glucosidase were investigated at different temperatures and pH values . Enzymatic activity for each enzyme was measured against its specific substrate, after incubation of the crude preparation in different temperatures ( 55,60,65 ) °c and different pH values (5.5,6.5,7.5); measurements were carried out at different time intervals (0,2,4,6,8,24)h . Results revealed that temperature 55°c and pH 6.5 representing the optimal conditions for thermostability of the crude enzyme; such that, the remained enzymatic activity for both endoglucanase and exoglucanase were 100% after 24 h of incubation, and 64.4% after 4 h of incubation for β-glucosidase at 60°c and pH 6.5 . Temperture 65°c and pH 7.5 shortened the half life of crude enzyme; such that, around 50% of the remained enzymatic activity was demonstrated after 6-8 h of incubation for both endoglucanase and exoglucanase, and not more than 2 h for β-glucosidase. | ||
| Keywords | ||
| Thermostability; Cellulases; Thermomonospora fusca | ||
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