THE EFFECTIVENESS OF THE BACTERIA PSEUDOMONAS FLOURESCENS AND RHIZOBIUM LEGUMINOSARUM AGAINST BEAN YELLOW MOSAIC VIRUS | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 45, Issue 6, September 2014, Pages 593-601 | ||
| Authors | ||
| S. M. Al-Mayahi; R. A. Al-Ani | ||
| Abstract | ||
| The study was conducted to evaluate the efficiency of Pseudomonas fluorescens and Rhizobium leguminosaruato induce systemic resistance in broad bean plants against bean yellow mosaic virus (BYMV). Broad bean seeds were submerged in suspension of R. leguminosarua, P. fluorescens and combined of them. plants emerged from these seed were inoculated with BYMV at three stages after germination. Plants height, foliage fresh weight, root fresh weight , foliage dry weight, root dry weight, Results showed that the disease incidence in plants emerged from seed treated with R.leguminosarua, P. fluorescens and combined of them were zero%. The decease incidence in plants emerged from seeds treated with the three above agents and inoculated with BYMV were 26, 25 and 20% respectively compared with zero% in control (healthy plant) and 92% in BYMV infected plants , and virus concentration in the plant, by DAS- Elisa using polyclonal antibodies for BYMV after 50 days of cultivation. High reduction in virus, concentration in plants from treated seeds and inoculated with virus was absurd. The absorbance values at 450 nm of extracts from plant treated with P. fluorescens, R.leguminosarum and combined of them 0.225, 0.240 and 0.150 respectively with inhibition percentages 59.75, 57.00 and 72.00% respectively compared with absorbance values 0.048 with extracts from healthy plants and in plants emerged from treated seeds non-inoculated with BYMV and 0.558 of extract from BYMV infected plants submersion of seed in bacterial suspension induced significant promotion in plant growth parameters. | ||
| Keywords | ||
| Nodulation; control; bacteria; BYMV | ||
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