DETECTION OF HEPATITIS B VIRUS USING ELISA AND REAL TIME PCR TECHNIQUES IN PATIENTS' BLOOD AND SALIVA IN AL- ANBAR PROVINCE | ||
| Journal of University of Anbar for Pure Science | ||
| Article 10, Volume 7, Issue 3, December 2013, Pages 55-59 PDF (396.88 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2013.94738 | ||
| Authors | ||
| AMIN S. BADAWY; THAER A. HASAN | ||
| Tikrit University - College of Agriculture | ||
| Abstract | ||
| :The present study has been carried out at the laboratories of the department of biology at the college of Education-University of Tikrit for the period from 1st Oct to 1st May 2012. The study consisted of the investigation about Hepatitis B in the saliva as compared with serum by using the ELISA and Real Time PCR techniques. Patients fallen asleep and revisers of the Ramadi General Hospital and the Fallujah General Hospital and who had confirmed cases hepatitis B for the purpose of pattern prove of saliva in the portability infection, where the results of the study were as follows: The results obtained showed that HBsAg antigen was found with 99.4% and (1.101± 0.335) mean ±SD in serum as compared with 48.6% in saliva with a (0.330±0.278) mean ±SD, that gave a noticeable increase when comparing with the control group ( 0.039 ± 0.024 ) and ( 0.017±0.014 ) for serum and saliva respectively. The value of T test for serum and control group equals( 16.25 ), while the value of T test for saliva and control group equals (4.18) which has high significance equals (0.000) with a test value equals ( P < 0.05). The proportion of correlation between saliva and serum in ELISA test equals (0.361) with high significance of test while the value of T test for them equals (28.902) with high significance of test with a test value equals ( P < 0.05). The genetic factor has been used in order to determine the levels of HBV DNA in the samples of positive saliva to test ELISA by using Real Time PCR . 95% from tested serum samples were positive to the viral load. While in saliva 50% from the samples HBV DNA was positive . | ||
| Keywords | ||
| Hepatitis B; ELISA; Real time PCR | ||
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