Determination of Anthocyanins Content in Prosopis farcta L. Callus Cultures | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 8, Issue 3, September 2014, Pages 59-63 | ||
| Authors | ||
| Rana Tariq Yahya; Hanaa Saeed Al-Salih | ||
| Abstract | ||
| The current study succeeded to initiate callus cultures of Prosopis farcta L. from hypocotyls stems which induced on the agar-solidified MS* medium supplemented with 4.0 mg/ L TDZ alone or with addition of 1.0 mg/ L NAA. Callus initiation percent reached 100% after 7 days of culture when hypocotyls stem segments were cultured on MS medium with addition of both TDZ and NAA. Whereas when TDZ alone were added initiation percent reached 60%. The results revealed detection of high amount of Anthocyanins which reached 440.98 µm/gm of fresh weight of callus cultures, grown on MS* medium supplemented with 1.0 mg/ L NAA and 4.0 mg/L TDZ, then 182.24 µm/gm of fresh weight of callus grown on MS with addition of 4.0 mg/L TDZ alone. MS* : MS medium with increasing KNO3 to 2000 mg/ L, Thiamine-HCl to 0.5mg/ L, Pyrodoxine-HCl to 1.0 mg/L | ||
| Keywords | ||
| Prosopis farcta plants; callus cultures; anthocyanins content | ||
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