RELATIONSHIP BETWEEN THE EXPRESSION OF CD34, CD123 AND MYELOPEROXIDASE MARKERS BY FLOW CYTOMETRY AND RESPONSE TO INDUCTION THERAPY IN ACUTE MYELOID LEUKEMIA | ||
| IRAQI JOURNAL OF MEDICAL SCIENCES | ||
| Article 1, Volume 12, Issue 2, August 2014, Pages 161-167 | ||
| Authors | ||
| Faez Sh. Almohsen; Subh S. Al-Mudallal | ||
| Abstract | ||
| Background:Different immunophenotypic markers were found to be related to the prognosis of acute myeloid leukemia. Among them are CD34, CD123 and myeloperoxidase. Objective:To evaluate the relationship between the expression of CD34, CD123 and myeloperoxidase markers by flow cytometry; and the initial response to induction therapy in acute myeloid leukemia patients. Method: A cohort of forty one patients with newly diagnosed de novo acute myeloid leukemia were prospectively tested for the expression of CD34, CD123 and myeloperoxidase using multicolor flow cytometry and re-evaluated for the response to a 7+3 induction therapy regimen. Results:It was found that 64.29% of CD123- patients achieved complete remission while 70.37% of CD123+ patients not (P = 0.035). For CD34, 55.56% of CD34- patients achieved complete remission while 63.64% of CD34+ cases not. The induction failure in CD34+M3 cases was 100% (P = 0.045). Regarding myeloperoxidase, 61.54% of patients who had >20% myeloperoxidase expression achieved complete remission while 70.37% (myeloperoxidase expression in <20% of cells) failed to achieve complete remission (P = 0.05). Conclusion:Expression of CD34 and CD123 and weak expression of myeloperoxidase (<20% of blast cells) are associated with poor response to induction therapy in acute myeloid leukemia patients. Keywords:Flow cytometry, CD34, CD123, myeloperoxidase, acute myeloid leukemia | ||
| Keywords | ||
| Flow cytometry; myeloperoxidase; acute myeloid leukemia | ||
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