Estimating genetic distance to variety of okra (Abelmoschus esculentus L.) by rapd marker | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 14, Issue 0, March 2014, Pages 110-119 | ||
| Authors | ||
| Ammar Hashim Saied; Majd Khleef AL-Kummer; Akeel Hussein AL-Asie | ||
| Abstract | ||
| This study was carried out during the growing seasons (2011/2012) at Vegetative Research Field, Department of Horticulture and Landscape design, College of Agriculture and Forestry, Mosul University . The objectives of this research was to study: the architecture of qualitative and quantitative traits of yield in okra plant ; combining ability , heterosis and correlation between the traits of the parents and first . Seven genetics line okra were used in this study namely (1- Clemson spineless, 2-Cleson, 3-Clemson spineless 80, 4-Sultani, 5-Samara, 6-Moslly and 7-Batra) . These genotypes were crossed in all possible combinations (Full Diallel Cross). The seeds of seven parents and their first including reciprocals were sown using Randomized Complete Block Design with three replications . The following traits were studies : estimate the molecular markers (fingerprint) of the parents and their first including reciprocal, at the laboratories of the molecular biology/Department of biology/College of Sciences/Tikrit University. The results can be summarized as follows :-Genomic DNA from 1.5- 2g of fresh tissue was isolated, a range yields were in the range of 300-500μL. DNA polymorphisms were scored within amplified bands on either agarose gels or electrophoresis according to DNA marker type. Data were further analysis further using the NTSYS-PC software to determine Genetic Distance (GD) and Genetic Relationship (GR) among barley varieties. The overall results obtained are as follows : In the RAPD experiments, 28 different primers were tested, only 20 primers produced amplified bands and the total number of these bands were 135.All them polymorphic bands. Only thirteen line were fingerprinted namely Local parents Cleson, Sultani, Moslly and Hybrid (1x2), (1x4), (1x5), (1x6), (2x4), (3x4), (3x6), (5x6), (3x7), The lowest (0.137) GD calculated was found between (7x2) and (6x4) and the highest (0.937) GD was found between (5) and (7x3) . RAPD – based markers molecular dendrogram (DG) showed two group clusters, the first included (6) parent and hybrid(1x2),(1x3),(1x4), (1x5), (1x6), (1x7), (2x3), (2x4), (2x5),the second included parent (5) the three groupincluded hybrid (2x6) , (2x7) , (3x4) , (3x5) , (4x6) , (4x5) , (6x7) , (5x7) , (4x7) , and the four clusters included (3x6),(3x7),(5x6) . | ||
| Keywords | ||
| Okra; variety | ||
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