Crystal Violet Binding Assay for Assessment of Biofilm Formation by Klebsiella pneumoniae on Catheter, Glass and Stainless-steel Surfaces | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 55, Issue 3, July 2014, Pages 1208-1212 | ||
| Authors | ||
| Rashid M. Musleh; Asmaa Q. Jebur | ||
| Abstract | ||
| In this paper, quantified study of the biofilm formed by Klebsiella pneumoniae isolated from urine specimen of patient suffering from acute urinary tract infection (UTI) on catheter, stainless-steel and glass coupon surfaces, as well as determine the relationship between time contact and biofilm progression using crystal-violet binding assay based on the values of optical density at 620nm of the crystal violet stain which bonded total biofilm biomass by resolubizing with 99.9% ethanol at the specific interval times. The result showed biofilm formed on three tested surfaces but in different degrees. According to obtained data, the catheter coupons presents a higher capability to attract bacteria cell and biofilm formation followed by glass surfaces while stainless-steel surfaces regard as a less attractive surfaces in bacterial adhesion and biofilm progression. The attachment of the bacterial cells on the fresh produce surfaces increase with the contact time but the increase reached a maximum at time 48h. in which, the optical densities of catheter, glass and stainless-steel coupon surfaces were (0.169 nm), (0.085 nm) and (0.07 nm) respectively. The statical analysis showed significant differences between substratum type's adherence and biofilm progression. | ||
| Keywords | ||
| biofilm; Klebsiella pneumoniae; Catheter; glass; Stainless; Steel; Crystal Violet Binding Assay | ||
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