The efficiency of Cinnamomum zeylanicum oil extract on some physiological and histological aspects in mice infected with hydatid cysts of Echinococcus granulosus | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 55, Issue 3, July 2014, Pages 1196-1107 | ||
| Authors | ||
| Ammal K. Abbas; Shatha K.Abbas; Areeg A.Zabbon | ||
| Abstract | ||
| The study aimed to determine the effect of the Cinnamomum zeylanicum oil extract on some aspects of physiological and histological in mice infected with larval stage of Echinococcus granulosus, and Assess the efficiency the Cinnamomum zeylanicum oil extract against experimental infection with protoscolices as of hydatid cysts . A total of 40 mice were divided into 4 equal groups. Each mouse in the first, second, and third groups were injected intraperitoneally (IP) with 2000 protoscolices, whereas mice in the fourth group were left as negative control (Injected with normal saline) non-infected control. Four months post infection, mice in the second and third group treated with concentrations (0.03 and 0.04 mg) of Cinnamomum zeylanicum oil extract respectively in every day for two week. Mice in the first group were untreated and considered as control group. Morphological and histopathological changes in cysts and infected organs were studied. Some blood parameter were measured including the Red and white blood count , pocket cell volume and the percentage of hemoglobin, liver enzymes also measured, including glutamate-oxaloacetate transaminase(GOT) and glutamate-pyruvate transaminase (GPT) . Level of sugar was also measured in the blood serum. Preliminary results in infected mice showed that the zeylanicum oil extract at both doses was effective in stopping the viability of protoscolices compared with the positive control mice. The study also showed a significant increase (P <0.05) in the number of red blood cells and the packet cell volume and hemoglobin percentage in the treated mice, especially those treated with a concentration of 0.04 mg compared to positive control mice . Also we record a significant decrease ( P <0.05) in the number of white blood cells in treated mice compared to control positive mice. Moreover record a significant decrease (P <0.05) in the enzymatic activity of the enzyme GOT and GPT and a significant increase (P <0.05) to the level of sugar in the blood of mice treated with extract of zeylanicum compared to the positive control mice. The results of the histological examination of the liver and spleen occurrence of infiltration and degeneration of the cells and the lack of cellular pattern of positive control mice compared with mice treated with zeylanicum oil extract at both concentrations. And it can be concluded that zeylanicum oil extract contains material studying at her efficiency in the effective elimination of primates initial in-vivo also have the ability to reduce some of the side effects of this parasite in vivo. It is hoped that the plant will be studying the importance of addressing the disease in hydatidosis an alternative to chemical treatments and surgeries. | ||
| Keywords | ||
| Cinnamomum zeylanicum; Hydatid cysts; Liver; Spleen; red blood cell; White blood cells; hemoglobin | ||
|
Statistics Article View: 67 PDF Download: 32 |
||