Evaluation of Peripheral Blood Lymphocytes as Cell Line for the Propagation of Human Herpes Simplex 1 | ||
| kufa Journal for Nursing sciences | ||
| Article 1, Volume 4, Issue 2, August 2014, Pages 18-24 | ||
| Author | ||
| Khalida K. Abbas Al-Kelaby | ||
| Abstract | ||
| Objectives: This study was planned to evaluate the use of peripheral blood lymphocytes as cell line for propagation of human herpes simplex 1, by the using of modern diagnostic techniques. Methodology: Primarily, 40 samples were collected from dermal lesions, investigated by RT-PCR technique directed to certify human herpes simplex1 infections Bosphore® HSV 1-2 Genotyping Kit v1(Anatolia geneworks,Turkey) was used for the detection protocol. Results: the results revealed that HSV1 was correlated with 23(57.5%) of the total cases investigated. Five of HSV1 positive samples were selected and applied to the assay of in vitro studying of specific cytopathic effects(CPE) via cell culture technique. Peripheral blood lymphocytes were isolated and propagated as a cell line. The demonstration of specific HSV1 cytopathic effect was demonstrated by indirect immunoflourescent antibody technique. This approach was revealed different degrees of sensitivity for supporting the growth of human herpes simplex1 virus; these cells were sensitive enough to support the growth of HSV1 virus. Conclusions: We concluded that Bosphore® HSV 1 Genotyping Kit v1allows very rapid detection of HSV DNA in dermal leisions. peripheral blood lymphocytes were efficient enough for the studying of CPE of HSV1, while PCR assay was more efficient and more précised as a diagnostic technique. Recommendations: Real-time polymerase chain reaction is complementary to cell culture technique in diagnosis of HSV, and it`s preferred to use specific primers of viral virulence factors and monitoring their pathogenicity. | ||
| Keywords | ||
| lymphocytes; cell culture; herpes simplex; Real; time PCR; fluorescent assay | ||
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