Direct Detection of Staphylococcus aureus from mastitis milk of cattle by SYBER Green dye based Real-Time PCR technique | ||
| Kufa Journal For Veterinary Medical Sciences | ||
| Article 1, Volume 5, Issue 1, June 2014, Pages 104-109 | ||
| Author | ||
| Fadi J. H. Al-Shammari | ||
| Abstract | ||
| The Real-Time PCR technique is a useful diagnostic tool for quick, high throughput and reliable routine screening of Staphylococcus aureus isolates. Moreover, this technique done by SYBR Green based quantitative detection of these pathogens in clinical samples such as mastitis milk of cattle. This study was conducted by Real-Time PCR technique to amplified highly conserved region 125bp fragment of 16S rRNA gene Staphylococcus aureus. The results show more frequency detection of Staphylococcus aureus in mastitis milk (95%). We concluded that Real-Time PCR technique provides a rapid and sensitive method for specific direct detection of Staphylococcus aureus in mastitis milk of cattle. | ||
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