Isolation and sero-diagnosis of Newcastle Disease Virus Infection in Human and Chicken Poultry Flocks In Three cities of Middle Euphrates | ||
| Kufa Journal For Veterinary Medical Sciences | ||
| Article 1, Volume 5, Issue 1, June 2014, Pages 16-21 | ||
| Authors | ||
| Ahmed M. Al-Shammari; Huda A. Al-Nassrawei; Ahmed Mahdi A. Kadhim | ||
| Abstract | ||
| This study was planned for detection of Newcastle Disease (ND) infection in human and chicken flocks in Euphrates by the using sero-diagnosis of heamagglutination (HA), heamagglutination inhibition assay (HAI) and competitive ELISA.The NDV was diagnosed in najaf chicken flock by isolation and propagation of virus in chicken egg embryos. The NDV Ab was detected in serum of local chicken flocks isolated by using competitive ELISA which was indicated the positive high Ab titer with inhibition percent titer more than 40%(PIt>40%) of the total samples. The fifty eight (58) collected human sera of most poultry associated people in Euphrates in Iraq was determined by NDV competitive ELISA and showed 3 cases from farmers were positive to NDV(PIt>40%). | ||
| Keywords | ||
| NDV; chicken egg embryos; HAI; competitive ELISA; PIt | ||
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