investigation for the effect of crude aqueous extract of Achillea Fragrantisime on the cytotoxicity of different cancer cell lines | ||
| Iraqi Journal of Cancer and Medical Genetics | ||
| Article 1, Volume 7, Issue 1, June 2014, Pages 86-91 | ||
| Authors | ||
| Zaid A. Ali; Noor T. Jafar; Aymn A. Hassan; Asma A. Almukhtar; Amal M. Ali | ||
| Abstract | ||
| In this study , investigation done on the effect of crude aqueous extract of Achillea Fragrantisime on the cytotoxicity of different cancer cell lines . This study containing three parts, first one including selection of four types of different cancer cell lines, two types of these are cancer cell line which are (AGM-3) and (AMN-3) and the other tow types are transformed which ( REF) and (VERO) for measuring the cytotoxicity of crude extract on them, The results showed that the effect of crude aqueous extract of this plant depends on the type of cell line concentration of extract and exposure time , it was found in cancer cell lines that the high concentrations of extract investigating the highest inhibition percentage of growth cell when compared with Control, while this percentage was less in inhibition of growth cell at low concentrations after 24 hours and then this percentage be lower after exposure for 48 hours, while after exposure for 72 hours there was no significant differences when compared with control. Same results where appeared in transformed cell line but the inhibition percentage was less from what is found in cancer cell line. Second part of this study has been measured the effect of plant crude extract on form and stimulate an apoptosis in cancer cell lines and that was happened through application of Tunnel assay technology and, the results appeared that this crude extract was stimulated on Apoptosis process in all used types of cancer cell lines . Finally, at the third part of this study was included measurement of genetic toxic effect of the plant crude extract through analysis micronucleus assey in human lymphocyte stimulated by Phytoheamaglotenine, and the Results appeared lack of MN which is one of certified analysis to investigation of breaking genetic material. The conclusion of the study that the crude aqueous extract of Achillea Fragrantisime was useful in inhibition of growth cancer cells, and increases stimulate appaptosis process , as well as it non toxic in normal lymphocytes and that make it outbalance to use it as alternative treatment to chemotherapy and radiotherapy habitual use in spite of there side effects and some disadvantage | ||
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