Determination of optimum condition for Poly-β-Hydroxybutyrate Production from Bacillus spp . | ||
| karbala journal of pharmaceutical sciences | ||
| Article 1, Volume 5, Issue 7, June 2014, Pages 183-194 PDF (0 K) | ||
| Authors | ||
| Najeh Hashem Kadhim and; Noor Abd- ulamer Oda | ||
| Abstract | ||
| The optimum cultural and ecological condition for Poly-β-Hydroxybutyrate Production from Bacillus spp. Isolate which was isolated from soil samples at holy Kerbala governorate were studied . These condition represented by the type and the concentration of carbon , nitrogen and mineral salts source ,in addition to the incubation period temperature , shaking speed , initial pH of production medium and occulation volume . Results revealed that , using production medium containing Date syrup at 2% as a source of carbon and ammonium chlorate at 0.2 % as a source of nitrogen , accompanied with Potassium phosphate and magnesium sulphate at 0.105 % as a total concentration for mineral salts source . The pH was adjusted to 7 with 2% v/v occulation volume . The medium was inoculated by using shaking inocular with 40 ˚C at 200 rpm /min for 72 hr. | ||
| Keywords | ||
| Polyhydroxybutyrates; Dates juice; Bacillus; Biopolymer | ||
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