Antimicrobial activity of ethanolic extracts of Raphanus sativus and Cyperus rotundus against some pathogenic bacteria and Candida albicans | ||
Baghdad Science Journal | ||
Article 1, Volume 11, Issue 2, April 2014, Pages 748-756 | ||
Authors | ||
Rasmiya H. Murad; Huda Suhail Abid; Alia Hussein Ali; Muna Turkey Al-mossawei | ||
Abstract | ||
The research was conducted to study the antimicrobial activity of ethanolic extracts with four concentration of each one (100,150,200,250 ) mg/ml of Raphanus sativus leaves and Cyperus rotundus roots were estimated against four types of pathogenic bacteria (Staphylococcus aureus , Bacillus cereus , Pseudomonas aeruginosa, Escherichia coli),also against Candida albicans ,by using agar well diffusion assay .The two extracts exhibited observing antimicrobial activity against all tested bacteria ,and this activity was increased with the increasing of concentrations belong to them .The highest effect of Raphanus sativus extract was against gram positive bacteria Staphylococcus aureus with mean zones of inhibition ranged between 9 mm (in 100 mg/ml concentration) to 26 mm (in 250 mg/ml concentration ),while the highest effect of Cyperus rotundus extract observed against gram negative bacteria Pseudomonas aeruginosa with mean zones of inhibition ranged between 17 mm (in 100 mg/ml concentration ) to 29 mm (in 250 mg/ml concentration ) , and the lowest effect of the two extracts observed against Escherichia coli . Furthermore , Cyperus rotundus extract did not showed any effective inhibition against Candida albicans in all concentrations , while Raphanus sativus extract showed highly effective inhibition against Candida albicans with mean inhibition zones ranged between 18 mm (in 100 mg/ml concentration) to 28 mm (in 250 mg/ml concentration ) . | ||
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