Genetic Discrimination among 21 Corn (Zeay mays L.) Varieties through Total Soluble Seed Protein Profile | ||
journal of kerbala university | ||
Article 1, Volume 10, Issue 0, January 2014, Pages 168-175 PDF (0 K) | ||
Authors | ||
Thamer Khadhair Merza; Attyaf Jmeel Thamir; Nidhal Abdul Hussein Al-Badeiry | ||
Abstract | ||
This study was conducted to investigate the ability of total soluble seed protein to discriminate among 21 of maize genotypes through using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). According to SDS analysis , a total of 118 amplified bands were obtained ranging in their molecular size 18-86KDa. Five out of main nine bands were polymorphic and four monomorphic with an average of polymorphism reaches 55.5%. Phylogenetic tree divided 21 of corn genotypes between two major groups each of them divided in to two subgroups .The first main group included 13 genotypes , while the second main group included eight genotypes . The ability of protein profile to give a distinctive pattern for a particular genotype could serve in future of studying corn seed response to different abiotic stress and studying genetic diversity . | ||
Keywords | ||
Cluster Analysis; SDS; ANALYSIS; Biochemical Markers; Corn | ||
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