Effect of rapid freezing and thawing on Malondialdehyde concentration , abnormal sperm chromatin percent and sperm parameters of Asthenozoospermic patients | ||
| kufa Journal for Nursing sciences | ||
| Article 1, Volume 4, Issue 2, August 2014, Pages 1-12 | ||
| Authors | ||
| Sahib Yahia Almoreshedy; Hisham Qassim Mohammad Al-Nowainy; Wafak Gabory Mohammad | ||
| Abstract | ||
| Objective : The study aimed to study the effect of rapid freezing and thawing on Malondialdehyde concentration and abnormal sperm chromatin percent and sperm parameters of Asthenozoospermic patients Methodology : This study was performed in the laboratory of Intra cytoplasmic Injection and freezing of sperm in the fertility center of Al-Sader Medical City / Al-najaf Alashraf City during the period from May, 2012 to April 2013 , The study included 30 of Asthenozoospermic patients, the statistical analysis of this study performed by using Student's t – test and paired t – test at the level of probability 0.05 Results : the results showed that is sperm rapid freezing process of patients who were Included in the study by used sperm freezing media ( Sperm Freeze) and doing the thawing processes after one month From freezing leads to a significant decreasing (p<0.05) In the sperm concentration, sperm progressive motility percent, sperm viability percent and normal sperm morphology percent. and a significant increasing (p<0.05) In The Malondialdehyde concentration and abnormal Sperm chromatin percent and no significant difference(p<0.05) In round Cell concentration in comparison with their ranges after activation Conclusion : It can be concluded From this study that sperm rapid freezing and thawing processes had a negative effects on the semen and sperm parameters and Malondialdehyde concentration and abnormal sperm chromatin percent In the all samples of study included patients . Recommendation: Adding Anti oxidants to the sperm freezing media to decrease the negative effects of freezing and increase the sperm parameters . | ||
| Keywords | ||
| effect; rapid freezing; thawing; Malondialdehyde; chromatin; Sperm parameters; Asthenozoospermic | ||
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