Immunomodulatory effects of Crocus sativus L. (petals) and Boragoofficinalis L. (whole plant) aqueous extracts in albino mice | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 25, Issue 1, March 2014, Pages 7-16 | ||
| Author | ||
| Zahraa Hussein Mohammed Kadri | ||
| Abstract | ||
| The present investigation aimed to evaluate the immunomodulatory effects C. sativus (petals) and B.officinalis (whole plant) aqueous extracts in albino mice. The parameters of evaluations were metaphase index (MI) of bone marrow and spleen cells, adenosine deaminase (ADA) specific activity of serum and Arthus (AR) and delayed-type hypersensitivity (DTH) reactions. The animals (32 mice) were distributed into four groups, each with eight mice. The first group was normal controls, which were orally administrated with 0.25 ml of distilled water. The second group included mice orally administrated with the immune suppressive drug cyclosporine (10 mg/kg). The third and fourth groups were orally administratedwith the crude extracts (48 mg/kg) of C. sativus and B. officinalis, respectively. The treatment was continued for 11 days as a single dose per day.The MI of bone marrow and spleen cells was significantly (P ≤ 0.05) decreased in mice treated with cyclosporine (10.88 and 6.58%, respectively), as compared with controls (16.63 and 10.38%, respectively) or mice treated with the extracts of C. sativus (21.80 and 13.96%, respectively) or B. officinalis(19.36 and 11.69%, respectively). An enhancement of AR and DTH reactions was also observed in mice treated with the extracts of C. sativus (1.13 and 1.38 mm, respectively) or B. officinalis(0.75 and 0.94 mm, respectively), and both means were significantly higher than the corresponding observed means in controls (0.44 and 0.52 mm, respectively) or cyclosporine-treated mice (0.39 and 0.41 mm, respectively).With respect to ADA, the highest specific activity was observed in mice treated with the extract of C. sativus (1.88 unit/mg protein), followed by mice treated with the extract of B. officinalis (1.56 unit/mg protein), controls (0.99 unit/mg protein) and finally cyclosporine-treated mice (0.35 unit/mg protein). | ||
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