DETECTION OF QUALITATIVE AND QUANTITATIVE ACTIVE COMPOUNDS IN THE LOCAL Trigonella foenum – graecum SEED | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 3, September 2010, Pages 385-396 | ||
| Abstract | ||
| The detection and quantitative determination of the active compound of Methanolic extract(80%)of local Trigonella foenum–graecum seeds were investigated by using high performance liquid chromatography(HPLC). It was found that the Methanolic extract contain many of active compounds such as Trigonellin (0.018%), Camphor (0.20%), Comarin (0.18%), Tripcin (1.9%), Vitexen (3.6%), Quercetin(1.21%) and Naringenin (0.27% ). The determination of fatty acid for fixed oil using the same technique showed that the percentage of Palmitic acid (24%) was the highest than the other fatty acids while the percentage of Aarchidic acid was the lowest (1%), the percentage of Olic acid and Linolenic acid were(12%, 9%) respectively. The thin layer chromatography technique (TLC) used for detection of diosgenin, with Rf equal to (0.7). The water extract cause agglutination of human red blood cells of groups (A, B,O) and this result provide found of Lectin in this extract, the titration percentage of agglutination was (64, 16, 16) respectively. | ||
| Keywords | ||
| Trigonella foenum; Active compond; HPLC | ||
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