Histopathological Effect of Partial Purification Aerolysin Extracted from Aeromonas hydrophila on Internal Organs of Experimental Mice | ||
| Medical Journal of Babylon | ||
| Article 1, Volume 11, Issue 1, March 2014, Pages 223-234 | ||
| Author | ||
| Iman Jawad Kadhim | ||
| Abstract | ||
| Eighty samples were collected from wound swabs, five isolates ( 6.25 %) were obtained and diagnosed as Aeromonas hydrophila. All strains had the ability to production of the aerolysin . The strain AH4 had highest hemolytic activity 1024 HU/ml. The aerolysin was extracted and purified partially by precipitation with ammonium sulphate (60%), the gel filtration was done by sephacryl S-300 with specific activity, numbers of purification and percentage of recovery reached 94208 unit mg protein, 1.363 times and 5 respectively, for the isolate AH4 which was the best in aerolysin production. The histopathological effect of the aerolysin on the internal organs (spleen, liver, kidney and lung ), the effect of the aerolysin was obvious in all histological samples.The histopathological observation results manifested that the damage of partial purification of aerolysin to the spleen, liver, kidney and lung of experimental mice is the most serious. | ||
| Keywords | ||
| Aeromonas hydrophila; Aerolysin; Histopathology | ||
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