Determination of Glucose Level as a Parameter for Cell Inhibition by 5-Flurouracil in Human Colonic Cancer SW-480 Cell Line | ||
| Medical Journal of Babylon | ||
| Article 1, Volume 11, Issue 1, March 2014, Pages 98-102 | ||
| Authors | ||
| Adeeb A. Al-Zubaidy; Najat A. R. Hasan; Hamid Naji Obeid | ||
| Abstract | ||
| Colonic cancer (SW-480) cell line was cultivated on two microtiter-plates of 48 wells, by using the routine culture methods and all what needed from culture media and reagents. One microtiter-plate of SW-480 cell line was exposed to serial double dilutions of 5FU starting from 80 µg/mL of 5FU for 12 hours incubation. Optical density was measured after staining with crystal violet and growth inhibition percentage was calculated. Second SW-480 cultured plate was also exposed to same 5-FU dilutions and incubation period, and then the glucose level was determined in the culture medium. There was significant increase (P<0.05) in the inhibition percent and glucose level in SW-480 culture media for 5FU concentrations starting from 2.5µg/ml and up word. In conclusion: According to the result, determination of glucose concentration may be a valuable predictor for the antitumor drug response. | ||
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