Spectrophotometric Determination of Thymol in Pharmaceutical Preparation via Diazotization Reaction with 4-aminoacetophenone | ||
| Rafidain Journal of Science | ||
| Article 5, Volume 25, Issue 3, June 2014, Pages 44-56 PDF (0 K) | ||
| DOI: 10.33899/rjs.2014.88567 | ||
| Author | ||
| Salih T. Al-Ramadhani | ||
| Abstract | ||
| A simple and sensitive spectrophotometric method is proposed for the determination of thymol. The method is based on the coupling reaction of the drug with diazotised 4-aminoacetophenone reagent in alkaline medium to produce an intense red coloured water -soluble and stable azo dye which exhibits a maximum absorption at 502 nm. Beer’s low is obeyed over the concentration range 0.2 – 16 µg/ml with a molar absorptivity of 2.89×104 L.mol-1.cm-1. The limit of detection is 0.0216 µg/ml and the limit of quantitation is 0.072 µg/ml, average recovery is 101.6%, and RSD is less than 2.4%. The proposed method is applied successfully to the determination of thymol in pharmaceutical preparation. | ||
| Keywords | ||
| Thymol; aminoacetophenone; Assay; spectrophotometry; diazotization; pharmaceutical preparations | ||
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