EFFECT OF IN VITRO PHENYL ALANINE APPLICATION ON ROSMARINIC ACID AND OTHER PHENOLIC COMPOUNDS ACCUMULATION IN Melissa officinalis | ||
| Euphrates Journal of Agriculture Science | ||
| Article 1, Volume 6, Issue 1, February 2014, Pages 57-66 | ||
| Abstract | ||
| Abstract : The plant tissue culture lab, of Horticulture dept. Abo Ghareeb - College of Agriculture - University of Baghdad, have witnessed our research work, which aims to make use of in vitro culture technology to evaluate the ability of increasing some plant phenolic compounds (secondary products) like (Rosmarinic, Caffeic, Coumaric and Hespertin) through expose the differentiated tissues (shoots) of Lemon Balm Melissa officinalis L. for a chemical phenylalanine elicitor. The in vitro multiplicities shoots which grown on MS media supplemented with 2 mg.L¹‾ BA and 0.2 mg.L¹‾ NAA where exposed to Different consecrations of phenylalanine 0.0,2.5,5,10 and 15 mg.L¹‾ , the results indicate that the MS which supplemented with 5 mg.L¹‾ Phenylalanine, give the significantly highest fresh weight 3688 mg, the significantly highest rosmarinic acid 477.0 µg.g¹‾ as compared with 100.44 µg.g¹‾ on the in vitro shoots and 26.94 µg.g¹‾ on the leaves from plant grown in green houses. | ||
|
Statistics Article View: 52 PDF Download: 1 |
||