The Effect of BA and NAA on Citrus Root stocks In Vitro | ||
| Euphrates Journal of Agriculture Science | ||
| Article 1, Volume 6, Issue 1, February 2014, Pages 48-56 | ||
| Abstract | ||
| A study was conducted at the tissue cultur Lab.College of Agriculture / University of Baghdad during 2012, to investigate the effect of plant growth regulators on micro propagation of three citrus root stocks Swingle Citrumelo, Troyer Citrange and Volka meriana . Either single nodal segment were investigated . Murashige and Skoog medium supplemented with different concentration of (BA, GA3, NAA ) were tested . In establishment stage , single nodal segment responded better on MS medium supplemented with (1.0 mg/ L) BA gave highest respond (100 %) while 0.3 GA3 gave (86.67%). In multiplication stage , MS medium supplemented with (1.5 mg/L) BA was the highest for shoot proliferation (11.2 shoot / explant) for Swingle, while MS medium supplemented with (1,2 mg / L )BA gave best results for Troyer citrang and Volkameriana (3.76, 8.2 shoot/ explant) respectively. Root stocks shoot gave (100%) rooting percentage at (1.0, 1.5, 2.0) mg/L NAA. Swingle shoots gave (11.9) root / shoot at (1.5 mg/ L) NAA . Troyer citrang gave the longest root (6.75 cm ) at 1 mg/L NAA . The acclimatized plantlets were (90 % , 90 % , 80 % ) for Swingle , Troyer and Volkameriana respectively when they planted on a composed consisted of 1:1 sand : peatmoss. | ||
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