Detection of the perfect condition to produce the tannase from Aspergillus niger at different medium | ||
| Journal of University of Babylon | ||
| Article 1, Volume 22, Issue 4, June 2014, Pages 1363-1371 | ||
| Authors | ||
| Myes E. Ahmed; Hind J.Abdal Rhman | ||
| Abstract | ||
| Three samples were collected to the test ability of tannase production from these isolates was studied semi quantitative and quantitative screening appeared that Aspergillus niger A2 was the highest tannase producer. Aspergillus niger A2 was grow in liquid medium under different available condition for tannase production were inoculation with 2.2x104 cell/ml of fungus in medium containing 1% (W/V) tannic acid at 25 C for 5 days. Tannase production reached 4 U/mg proteins in these conditions; tannase was precipitation from production media by 80% saturation of Ammonium sulphate with 4.2 U/mg protein. The enzyme obtained from this step was particularly purified with 1.3 fold of purification and 62.6 yield. | ||
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