Antibacterial Effects of Lactic Acid Bacteria Cell Free Extract on the Growth of Local Isolates of Listeria moncytogenes from some Food Sources | ||
| Rafidain Journal of Science | ||
| Article 3, Volume 25, Issue 1, February 2014, Pages 19-30 PDF (0 K) | ||
| DOI: 10.33899/rjs.2014.86055 | ||
| Authors | ||
| Zainab M. Alzubaidy; Shean I. Kakey; Jassim F. Ali | ||
| Abstract | ||
| The present study aimed to determine the antibacterial activity of lactic acid bacteria metabolites against Listeria monocytogenes which was isolated from some foods in local markets of Erbil and Koya cities; 225 food samples were examined for the presence of Listeria species particularly L. monocytogenes from October 2010 to March 2011. The studied samples included 70 samples of raw chicken meat, 55 samples of raw milk, 50 samples of cheese and 50 samples of raw red meat. According to motility test, hemolysin production, sugar fermentation test, 8 isolates of L. monocytogenes were identified, 1 (2 %) isolate from cheese, 2 (4 %) isolates from red meat and 5 (7.3 %) isolates from chicken meat. The percentages of contaminated foods for Listeria species from chicken meat, raw milk, cheese and red meat were 20, 9, 20 and 22% respectively. With respect to the biocontrolling attempt, the effect of cell free extract (CFE) of lactic acid bacteria (LBA) which is considered as safe against isolated L. monocytogene was used as biocontrolling agent. The CFE of lactic acid bacteria which isolated from local dairy products, was tested for its controlling ability by agar diffusion method, the CFE showed inhibition zone from (15-23 mm) in diameter. Also the minimum inhibitory concentration MIC of CFE was determined at three additive percentages (0.5, 1.0 and 1.5ml to 10 ml of nutrient broth), the inhibition percentage ranged from (28.75 - 48.97) at (0.5 %), (52.13-95.55) at (1 %) and (82.93 -98.15) at (1.5 %) additive percentage of CFE of LAB. | ||
| Keywords | ||
| Listeria moncytogenes; Lactic Acid Bacteria | ||
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