Molecular genetic study of Pseudomonas aeruginosa DNA repair system
|Journal of University of Anbar for Pure Science|
|Article 13, Volume 7, Issue 1, April 2013, Pages 49-56 PDF (517.65 K)|
|Document Type: Research Paper|
|Waleed Khalid Mohammed*|
|University Of ALanbar - College of Dentistry|
|Bacteria Pseudomonas aurginosa, E coli and Stapthylococcus aureus were exposure to different doses of ultraviolet radiation and survival curves drawn for each type, the results show that the bacteria Pseudomonas aurginosa more resistant by UV radiation than Ecoli and Stapthylococcus aureus bacteria. The bacterium Pseudomonas aeruginosa was irradiated with different doses of U.V light via wave length( 254 nm ) for different periods ( 50 , 100 , 150 , and 200 sec ) .It appear that part of irradiated bacterial culture was exposed to sun light and the other part was kept in the dark . The survivors of the cells exposed to the sun light was more than the dark and this ensure possessing the bacterium photoreactivating repair system investigate the excision repair system, the minimal inhibitory concentration ( MIC) of caffeine against bacteria was studied by exposing the bacterium to different concentrations of caffeine (10 , 15 , 20 and 25 mg/ml ) and the MIC was 20 mg/ml , Furthermore the bacterium was exposed to different times of U.V. light in the presence of caffeine and the studying ensure that the survivors of the cells in the medium with caffeine was less than the medium with absence of caffeine and this leads to possess the bacterium excision repair system. To detect the recombination repair system , the bacterium was exposed to the concentrations( 0.1 ,0.2 , 0.3 , 0.4 μg / ml) of acrivlavine and the MIC was 0.3 μg / ml , then the bacterium was exposed to different times of U.V. light in the presence of acrivlavine . The survivors of the cells in the medium with acrivlavine was less compared with the absence of acrivlavine. It would seem that possessing bacterium recombination repair system . sensitivity test of the bacterium against antibiotics was established and the results appeare that it was to the antibiotics Chloramphenicol, Carpencillin, Trimethoprim, Rifampicin The diameters of inhibition were (16,20,17,18) mm respectively and resistant to the antibiotics Amoxicillin, Ampicillin, Clindomycin, Cloxacillin , Nalidixic acid , Cephaloxin , Tetracyclin and Tobromycin. To study SOS repair system the bacterium was mutated with direct mutagens represented with nitrous acid and indirect mutagens represented with U.V. light to isolate Rifampicin and Chloramphenicol mutants. It is quite likely that the sensitivity of bacterium for mutagenesis then possessing SOS repair system .|
|Molecular genetic; Pseudomonas aeruginosa; DNA repair system|
1- Zhiwei Fang,b Jiao Zhao,b Yuanqiang Zou,b Tianzhi Li,a Junfeng Wang,a Yinghua Guo,a De Chang, a Longxiang Su,a Peixiang Ni,b and Changting Liua (2012). Draft Genome Sequence of Pseudomonas aeruginosa Strain ATCC 27853.American Society for MicrobiologyVolume 194 Number 14 Journal of Bacteriology p. 3755
2- Balcht, Aldona & Smith, Raymond (1994). Pseudomonas Aeruginosa: Infections and Treatment. Informa Health Care. pp. 83–84. ISBN 0-8247-9210-6.
3- Horst, J.P., Wu, T.H. and Marinus, M.G. (1999) Escherichia coli mutator genes. Trends Microbiol. 7, 29-36.
4- Miller, J.H. (1996) Spontaneous mutators in bacteria: insights into pathways of mutagenesis and repair. Annu. Rev. Microbiol. 50, 625-643.
5- Thiagarajan, M. Byrdin, A.P.M. Eker, P. Müller & K. Brettel (2011). "Kinetics of cyclobutane thymine dimer splitting by DNA photolyase directly monitored in the UV". Proc. Natl. Acad. Sci. USA 108: 9402–9407.
6- , C., Thaler, D.S., and Radman, M. (1989) The barrier to recombination between Escherichia coli and Salmonella typhimurium disrupted in mismatch-repair mutants. Nature 342: 396–401
7- Matic, I., Radman, M., Taddaei, F., Picard, B., Doit, C.,Bingen, E., et al. (1997) High variable mutation rates in commensal and pathogenic Escherichia coli. Science 277:1833–1834.
8- J . W. & MOUNTD, . W. (1982). The SOS regulatory system of Escherichia coli. Cell 29, 11-22.
9- WALKER, G. C. (1984). Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiologiccll Rwiews 48, 60-93.
10- Sancar A. (2003). "Structure and function of DNA photolyase and cryptochrome blue-light photoreceptors". Chem Rev 103 (6): 2203–37.
11- Clark AJ, Margulies AD. Isolation and characterization of recombination-deficient mutants of Escherichia coli K12. Proc Natl Acad Sci U S A.1965; 53:451-459.
12- Kuzminov A. Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda. Microbiol Mol Biol Rev 1999; 63(4):751-813.
13- Horii T, Ogawa T, Nakatani T, Hase T, Matsubara H, Ogawa H.Regulation of SOS functions: purification of E. coli LexA protein and determination of its specific site cleaved by the RecA protein.Cell.1981; 27(3 Pt 2):515-522.
14- Little JW, Mount DW. The SOS regulatory system of Escherichia coli. Cell. 1982; 29(1):11-22.
15- Walker GC. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev. 1984;48(1):60-93.
16- Aeschlmann (2003). The role of Multidrug Efflux Pumps in the Antibiotic Resistance of Pseudomonas aeruginosa and other Gram negative bacteria. Pharmacotherapy 22(7): 916-924.
17- Al-Dolaimi KJ(2012). Detection DNA repair system of the bacteria pseudomonas aeuroginosa. Journals anbar university of pure science .6 (1) ISSN 1991-8941.
18- Dougals, m.w.; perani, Aand Routh, (1995). Ultraviolet exposure studies on agamma radiation resistant Micrococcus isolated from food. Food Res ,243.376-382.
19- Piyush Tripathi, Gopa Banerjee, Shivani Saxena, Mahendra Kumar Gupta, andP. W. Ramteke (2011). Antibiotic resistance pattern of Pseudomonas aeruginosa isolated from patients of lower respiratory tract infection, African Journal of Microbiology Research Vol. 5(19), pp. 2955-2959, ISSN 1996-0808 ©2011 Academic Journals.
20- Jordi Barbe, Isidre Gibert, Montserrat lagstera and Riccardo Guerrero (1987). DNA Repair Systems in the Phototrophic Bacterium Rhodobacter capsulatus. Journal of' General Microbiology, 133, 961 -966. Printed in Great Britain.
21- A.Campbell and R.E.Yasbin (1984). A DNA Excision Repair System for Neisseria gonorrheae. Mol Gen Genet, 193:561-563.
22- Celina Janion (2008). Inducible SOS Response System of DNA Repair and Mutagenesis in Escherichia coli. int J Biol Sci, 4(6): 338–344.
23- National Committee for Clinical Laboratory Standards(2002). Performance Standards for antimicrobial susceptibility testing. 8th Infor-mational Supplement. M100 S12. National Com-mittee for Clinical Laboratory Standards,. Villa-nova, Pa.
Article View: 65
PDF Download: 31