Effect of dietary supplementation with rocket salad (Eruca sativa) seeds powder on testis histological traits of roosters subjected to oxidative stress induced by hydrogen peroxide | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 8, Issue 1, March 2014, Pages 18-26 | ||
| Authors | ||
| Hazim J. Al - Daraji; R. H. Razuki | ||
| Abstract | ||
| This study was conducted to evaluate the effect of adding rocket salad seeds powder to the diet on testis histological traits of roosters subjected to oxidative stress induced by hydrogen peroxide. A total of 60 Hy – line laying breeder roosters 57 weeks old were used in this study. Roosters were randomly distributed on 5 treatments with 3 replicates each treatments were as follows: T1: males were fed control diet and normal water, T2, T3, and T4: males were fed control diet supplemented with 3 gm rocket salad powder / kg of diet + 0.25 ml hydrogen peroxide 0.5%/ litter of water, 0.5 ml hydrogen peroxide 0.5%/ litter of water, and 1 ml hydrogen peroxide 0.5%/ litter of water, respectively. The T5: males were fed control diet and drank water supplemented with 1 ml hydrogen peroxide 0.5%/ litter of water. Males were treated with hydrogen peroxide 6% and rocket salad for 12 weeks starting from 59 week of ages. Results revealed that water supplementation with hydrogen peroxide without adding rocket salad powder to the diet (T5) resulted in significant deterioration as regards testis weight, measurements of seminiferous tubules, and volume density and relative weight of active components of seminiferous tubules and interstitial tissue as compared with T1 group. However, supplementing the diet of roosters subjected to oxidative stress induced by hydrogen peroxide with rocket salad seeds powder (T2, T3, and T4) resulted in significant improvement concerning histological traits involved in this experiment in comparison with T5 group. In conclusion adding rocket salad powder to the ration of roosters could limit to a considerable extent the damages on histology of the testis that originate from oxidative stress. Therefore, rocket salad powder could be used as one of active tools for improving reproductive performance of roosters. | ||
| Keywords | ||
| rocket salad seeds; Oxidative stress; histological traits | ||
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